From 200 gigabytes
to 0.5 millilitres
Read one dog's tumour and blood whole, and you get 200 gigabytes. Five months later that file had become 0.5 millilitres of clear liquid in a syringe.
These 20 lessons take apart, in the order the story tells them, every term wedged in between โ somatic variant calling, Mutect2, VEP, RMSD, OpenMM, NetMHCpan, sequence design, IVT and RIN, SM-102, LNP, DLS, endosomal escape. Python runs right inside this page. There is nothing to install.
How you'll learn
The order is the novel's, not a textbook's. Read lesson 3 and chapter 6 opens up; read lesson 16 and chapter 11 does.
VAF 0.847, RMSD 3.87 ร , IC50 87 nM, RIN 8.4, apparent pKa 6.41, 88 nm. Not one invented value. We work out why each number is the size it is.
The first LNP failed at 210 nm; the second passed every QC check and still failed inside the cell. You learn nothing from the batch that worked.
Every lesson has a laboratory widget โ the VAF threshold, the DFG angle, the pKa slider, the flow-rate ratio โ turned by you, so you see the conclusion flip.