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Prof. Jeong's mRNA Vaccine Design, First Steps II

Twenty fragments,
one protein

Join the twenty epitopes from Part I and you get a single 419-residue protein. This part covers only that joining: what holds the pieces together, what else gets attached, and what shape the result takes.
The paper gives the construct's sequence only as a figure, so we rebuild it ourselves. Three numbers prove the rebuild is right — length 419, mass 44.37 kDa, isoelectric point 9.56, all matching the paper to the last digit.
With that ruler in hand you can catch the 42 residues missing from the text, and the epitope that is one residue short, for yourself.
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🐍 Python🧊 construct🔗 linkers 📐 cross-checks🪤 pitfalls📊 quality metrics🇬🇧 English

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How you'll learn

🧊Build it block by block

The paper gives the sequence only as a figure. You rebuild it by hand; hitting 419 means you got it right.

📐Check it with three rulers

Length, mass, isoelectric point. Each measures something different, so when all three agree little room is left for error.

🪤Catch mismatches with arithmetic

The 42 missing residues and the epitope that is one residue short both show up in the numbers first.

📊Ask what each metric measures

Half-life, solubility, Ramachandran, MolProbity — each measures less than you would expect.

Full curriculum

에피토프 스무 개를 한 줄로 잇는다

Fig 1A · Methods 4

링커 넷 — AAY · GPGPG · KK · EAAAK

Methods 4 · Discussion

프로테아좀이 링커를 자른다는 말

Discussion · 링커 선택의 근거

β-디펜신 II — 보조제를 앞에 붙이는 까닭

Methods 4 · Discussion · 41잔기

구조물을 손으로 짓는다

Fig 1A · Fig 1B · Table 4 로 맞춘 복원

분자량 44.37 kDa 을 검산한다

Results 2 · ProtParam

본문에 없는 42잔기

Methods 4 vs Fig 1A — 우리가 센 것

Table 2 보다 한 잔기 짧은 에피토프

Table 2 vs Fig 1B · 산수가 가른다

범례가 TAT 라 부르는 꼬리

Fig 1A 범례 · 409–419

링커가 에피토프 안에 들어 있을 때

Table 1 · Table 2 — 우리가 센 것

ProtParam 의 수치 여섯 — 계산과 표 조회

Results 2 · ProtParam

반감기 30시간은 아미노산 한 개를 보고 적은 값이다

Results 2 · N-end rule

등전점 9.56 과 용해도 0.515

Results 2 · ProtParam · Protein-Sol

2차 구조 — 나선 31 · 가닥 25.5 · 고리 43.4

Results 3 · Fig 1B · PSIPRED 4.0

3차 구조 — Robetta 는 무엇을 하나

Results 3 · Fig 2A · Robetta / RoseTTAFold

GalaxyRefine 과 MolProbity 2.006

Results 3 · Fig 2B · Fig 2C

라마찬드란 90.1% 는 어느 자로 재느냐에 달렸다

Results 3 · Fig 3B · PROCHECK

ProSA −7.54 와 ERRAT 93.052 — 셋은 다른 것을 잰다

Results 3 · Fig 3C · 3D · 3E

실습 종합 — 입체 B세포 에피토프 넷을 구조물 위에 얹는다

Results 4 · Table 4 · Fig 4 · ElliPro

말할 수 있는 것과 말할 수 없는 것

II 권 정리 · Limitations