You switched off ten thousand genes.
Which one is the target?
If the answer were “whatever moved the most”, you would not need this course. It is not. The genes that move a cell most are usually the genes every cell needs — that is toxicity, not a target.
A target reverses the disease signature, leaves healthy cells alone, has somewhere to grab, and has human genetics on its side. Over these 20 lessons you build those four filters yourself and push 135 real CRISPRi screen results through them: 135 → 129 → 115 → 103 → 87 → 54. Python runs right inside this page.
How you'll learn
Not the gene that moves the cell most, but the one that turns the disease signature around. You build the connectivity-map style score by hand.
False hits from the cell cycle, false negatives from weak knockdown, toxicity climbing the ranking when you sort by effect size — you meet each trap with your own hands.
The actual results for 135 genes from a human primary CD4+ T-cell CRISPRi screen, plus DepMap's 1,247 common-essential genes. Not a stand-in dataset.
Druggability and human-genetics scores are not in this dataset. They are left blank rather than invented — with a note on where to get them.