This lesson is only available in Korean for now.
Dr. Yoo's mRNA Vaccine Synthesiser Cost Analysis, First Steps

The one number
the paper omits

The paper tells you there are seven peristaltic pumps, six valves and eleven reservoirs. It gives you ramp rates and flow ratios. It never gives you a single price.
This course unfolds the machine from /msyn and the reagents from /reag into one bill of materials and prices every line at today's rates. Lines we could not price stay blank โ€” we do not invent them.
Python runs right inside this page. There is nothing to install.

๐Ÿ Python๐Ÿ“‹ BOM๐Ÿชœ three boundaries ๐Ÿ”Ž quote-only๐Ÿ“‰ amortisation๐Ÿญ RUO vs GMP๐Ÿ‡ฌ๐Ÿ‡ง English
Start lesson 1 โ†’

How you'll learn

๐Ÿ“‹Prices onto counts

Beside the counts the paper does give, we add unit price, subtotal, source and date. That is a BOM.

๐ŸชœBoundary first

The box alone, plus instruments, or starting from an empty room. A total without its boundary misleads.

๐Ÿ•ณ๏ธBlanks stay blank

Quote-only items, an unnamed lipid. We never write zero โ€” we count the blanks instead.

โš–๏ธOnly the heavy lines

Four lines carry 73% of a batch. Saving on all the rest moves only the last quarter.

Full curriculum

01
๋…ผ๋ฌธ์— ์—†๋Š” ์ˆซ์ž ํ•˜๋‚˜ โ€” ๊ฐ’
Wei 2023 ยท ๋‘ ๋…ผ๋ฌธ ์–ด๋””์—๋„ ์›๊ฐ€ ์ž๋ฃŒ๊ฐ€ ์—†๋‹ค
โœ“
02
๋ถ€ํ’ˆํ‘œ โ€” ํ•œ ์ค„์— ๋ฌด์—‡์„ ์ ๋Š”๊ฐ€
BOM ์˜ ์ผ๊ณฑ ์นธ, ๊ทธ๋ฆฌ๊ณ  โ€œ๊ฒฌ์  ๋ฌธ์˜โ€ ๋ผ๋Š” ์นธ
โœ“
03
๊ฒฝ๊ณ„ โ€” ์–ด๋””๊นŒ์ง€๋ฅผ ์›๊ฐ€๋ผ๊ณ  ๋ถ€๋ฅผ ๊ฒƒ์ธ๊ฐ€
๊ฐ™์€ ์ƒ์ž๋ฅผ ๋‘๊ณ  ๋‹ต์ด ์…‹์œผ๋กœ ๊ฐˆ๋ฆฌ๋Š” ๊นŒ๋‹ญ
โœ“
04
์—ฌ์„ฏ ๊ฑธ์Œ์ธ๋ฐ ๋ชจ๋“ˆ์€ ๋‘˜ โ€” ์›๊ฐ€๊ฐ€ ๊ฑธ์Œ ์ˆ˜๋ฅผ ๋”ฐ๋ผ๊ฐ€์ง€ ์•Š๋Š”๋‹ค
Wei 2023 ยท Fig. 2 โ€” ํ•œ ๋ชจ๋“ˆ์ด ๋„ค ๊ฑธ์Œ์„ ํ•œ๋‹ค
โœ“
05
์˜จ๋„ โ€” ๋งŒ ์›์งœ๋ฆฌ ์†Œ์ž๋กœ 1.8 โ„ƒ/s ๋ฅผ ๋งŒ๋“ ๋‹ค
ํŽ ํ‹ฐ์— ยท PT-1000 ยท ์ปจํŠธ๋กค๋Ÿฌ ยท ๋‘ ๋ฒŒ ยท 2026-09 ์‹œ์žฅ๊ฐ€
โœ“
06
์•ก์ฒด โ€” ํŽŒํ”„ ์ผ๊ณฑ๊ณผ ๋ฐธ๋ธŒ ์—ฌ์„ฏ์ด 6๋งŒ์›
์—ฐ๋™ํŽŒํ”„ 7 ยท 3๋ฐฉ ๋ฐธ๋ธŒ 4 ยท 5๋ฐฉ ๋ฐธ๋ธŒ 2 ยท ํŠœ๋ธŒ์™€ ํ”ผํŒ…
โœ“
07
์ž์„ โ€” 1,250์›์ด 119๋งŒ์›์„ ๋Œ€์‹ ํ•œ๋‹ค
N42 ์›ํŒ์ž์„ + ์Šคํ…Œํผ๋ชจํ„ฐ + 3D ์ถœ๋ ฅ ๊ป๋ฐ๊ธฐ ยท DynaMag-2 ์™€ ๊ฒฌ์ฃผ๊ธฐ
โœ“
08
ํ—ค๋ง๋ณธ ์นฉ์€ 6๋งŒ์›์ธ๋ฐ, ๊ทธ ์นฉ์„ ๋ฏธ๋Š” ์žฅ๋น„๋Š” ๊ฐ’์ด ์—†๋‹ค
Fluigent ์••๋ ฅ ์ œ์–ด๊ธฐ ยท NanoAssemblr โ€” ๋‘˜ ๋‹ค ๊ฒฌ์ ์ œ
โœ“
09
์ œ์–ด โ€” ๋…ผ๋ฌธ์— ํ•œ ์ค„๋„ ์—†๋Š” ๋ถ€ํ’ˆ์— ๊ฐ’์„ ๋งค๊ธด๋‹ค
ํ„ฐ์น˜์Šคํฌ๋ฆฐ ยท ๋ณด๋“œ ยท ์ „์› ยท ํ”„๋ ˆ์ž„ ยท 3D ์ถœ๋ ฅ
โœ“
10
ํ•ฉ๊ณ„ โ€” 31๋งŒ์› ยท 68๋งŒ์› ยท 900๋งŒ์›
์ง์ ‘ ๋งŒ๋“ค๊ธฐ ยท ์นฉ๊นŒ์ง€ ยท ์ƒ์šฉ ์žฅ๋น„๋กœ ๊ฐ–์ถ”๊ธฐ
โœ“
11
์žฌ๋Š” ๊ฐ’์€ ๋งŒ๋“œ๋Š” ๊ฐ’๋ณด๋‹ค ๋น„์‹ธ๋‹ค โ€” ๋‹ค๋งŒ ์ฆ๋ช…ํ•˜์ง€ ๋ชปํ–ˆ๋‹ค
Bioanalyzer ยท DLS ยท TEM ยท ํ˜•๊ด‘ํ˜„๋ฏธ๊ฒฝ โ€” ์ •๊ฐ€๋ฅผ ํ•˜๋‚˜๋„ ์ฐพ์ง€ ๋ชปํ–ˆ๋‹ค
โœ“
12
์‹œ์•ฝํ‘œ์— ๊ฐ’์„ ๋ถ™์ธ๋‹ค โ€” ์„œ๋ฅธ ๊ฐ€์ง€์— ๋‹จ๊ฐ€ ์นธ์„ ๋”ํ•˜๊ธฐ
/reag ์˜ ์‹œ์•ฝ ์„œ๋ฅธ ๊ฐ€์ง€ ยท 2026-09 ์นดํƒˆ๋กœ๊ทธ ์ •๊ฐ€
โœ“
13
ํšจ์†Œ ๋„ท โ€” ํ‚คํŠธ๋กœ ์‚ฌ๋ฉด ์ผ๊ณฑ ๋ฐฐ๊ฐ€ ๋œ๋‹ค
LA Taq ยท T7 ยท DNase ยท E-PAP ยท 2026-09 ์ •๊ฐ€
โœ“
14
์žฌ๋ฃŒ๊ฐ’ โ€” ์บก์ด Cap 1 ์ธ๋ฐ ๋” ์‹ธ๋‹ค
rNTP 1.17 ยตmol ยท ์บก ์œ ์‚ฌ์ฒด 0.075 ยตmol ยท 2026-09 ์ •๊ฐ€
โœ“
15
์ง€์งˆ ๋„ท โ€” ์ด๋ฆ„์ด ์—†์œผ๋ฉด ๊ฐ’๋„ ์—†๋‹ค
๋…ผ๋ฌธ ํ‘œ๊ธฐ๋Š” โ€œlipid vectorsโ€ ํ•œ ๋งˆ๋”” ยท ํ‘œ์ค€ ๋ชฐ๋น„ 50:10:38.5:1.5 ๋กœ ๋Œ€์‹  ๊ณ„์‚ฐ
โœ“
16
๊ฐ€์žฅ ๋ฌด๊ฑฐ์šด ์ค„์€ ํšจ์†Œ๊ฐ€ ์•„๋‹ˆ๋ผ ๋น„๋“œ์˜€๋‹ค
oligo d(T) ๋น„๋“œ 200 ยตL ยท ์ŠคํŠธ๋ ™ํƒ€๋น„๋”˜ ๋น„๋“œ 1 mg(๊ฐ€์ •) ยท ์žฌ๋Š” ์‹œ์•ฝ
โœ“
17
ํ•œ ๋ฐฐ์น˜ โ€” 22๋งŒ์›๊ณผ 34๋งŒ์› ์‚ฌ์ด
300 ยตg ๊ธฐ์ค€ ยท ๋‘ ๊ฐ€์ง€ ๊พธ๋ฆผ ยท 1 ยตg ๋‹น 686 ~ 1,711 ์›
โœ“
18
ํ•œ ํšŒ๋ถ„์— ์–ผ๋งˆ์ธ๊ฐ€ โ€” ๋…ผ๋ฌธ์€ ์ด ๊ณ„์‚ฐ์„ ํ•˜์ง€ ์•Š์•˜๋‹ค
๊ฐ๊ฐ€์ƒ๊ฐ ยท ๊ฐ€๋™๋ฅ  ยท ๊ทธ๋ฆฌ๊ณ  โ€œํšŒ๋ถ„โ€ ์ด๋ผ๋Š” ๋ง์˜ ๋‘ ๊ฐ€์ง€ ๋œป
โœ“
19
ใ€์‹ค์Šตใ€‘ ์˜ˆ์‚ฐ์„ ๋„ฃ์œผ๋ฉด ๊ตฌ์„ฑ์ด ๋”ฐ๋ผ ๋‚˜์˜จ๋‹ค
3๋…„ ์ด์†Œ์œ ๋น„์šฉ ยท ๊ฐ€๋™๋ฅ  ยท ๊ทธ๋ฆฌ๊ณ  ๋ฌด์—‡์„ ํฌ๊ธฐํ•  ๊ฒƒ์ธ๊ฐ€
โœ“
20
๋งํ•  ์ˆ˜ ์žˆ๋Š” ๊ฒƒ๊ณผ, ๋นˆ์นธ ์—ด์ผ๊ณฑ
์ด ์›๊ฐ€ํ‘œ๋กœ ์–ด๋””๊นŒ์ง€ ๋งํ•  ์ˆ˜ ์žˆ๋‚˜
โœ“